It is important to define your cell preparations
DNA-methylation patterns of cells in culture reflect the tissue of origin, the cell type, and culture conditions. Cygenia offers the following assays to provide epigenetic quality control of your cells in culture:
In vitro culture is associated with very specific DNA-methylation changes that are continuously acquired during culture expansion. Some of these can be used to track the state of cellular aging and reflect the number of previous passages.
The pluripotent state of induced pluripotent stem cells (iPSCs) and of embryonic stem cells (ESCs) can be validated by an epigenetic signature.
Classification of cell preparations into mesenchymal stromal cells vs. fibroblasts.
Classification of mesenchymal stromal cells derived from bone marrow versus adipose tissue.