Analysis of Biological Age

• Han Y, et al. Epigenetic age-predictor for mice based on three CpG sites. Elife, 2018; 7. pii: e37462.

• Sheng C, et al. A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation. Nature Communications, 2018, 9: 4047.

• Frobel J, et al. Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice. Clinical Epigenetics, 2018, 10: 76.

• Fernandez-Rebollo E, et al, Primary osteoporosis is not reflected by disease-specific DNA. Journal of Bone and Mineral Research, 2018, 33: 356-361

• Zhang Y, et al. Individual CpG Sites that are Associated with Age and Life Expectancy become Hypomethylated upon Aging. Clinical Epigenetics, 2017, 9: 9.

• Wagner W. Epigenetic aging clocks in mice and men. Genome Biology, 2017, 18: 107.

• Eipel M, et al. Epigenetic age predictions based on buccal swabs are more precise in combination with cell type-specific DNA methylation signatures. Aging 2016; 8 (5):1-5.

• Lin Q, et al. DNA methylation levels at individual age-associated CpG sites can be indicative for life expectancy. Aging 2016; 8: 394-401.

• Lin Q, Wagner W. Epigenetic Aging Signatures are Coherently Modified in Cancer.  PLoS Genetics 2015; 11: e1005334.

• Weidner CI, et al. Epigenetic aging upon allogeneic transplantation: the hematopoietic niche does not affect age-associated DNA methylation. Leukemia 2015 [Epub ahead of print]

• Wagner W, Weidner CI, Lin Q. Do age-associated DNA methylation changes increase the risk of malignant transformation? Bioessays. 2015; 37:20-24.

• Weidner CI et al. Aging of blood can be tracked by DNA methylation changes at just three CpG sites. Genome Biology, 2014; 15:R24.

• Weidner CI, Wagner W. The Epigenetic Tracks of Aging. Biol. Chem. 2014; 395:1307-14.

• Fuellen G et al. In-Silico-Approaches and the Role of Ontologies in Aging Research. Rejuvenation Res. 2013; 16:540-546.

• Horvath S. DNA methylation age of human tissues and cell types. Genome Biol. 2013;14(10):R115.

• Koch C and Wagner W. Epigenetic-Aging-Signature to determine age in different tissues. Aging 2011; 3: 1-10.

• Koch C*, Suschek CV* et al. Specific age-associated DNA methylation changes in human dermal fibroblasts. PLoS ONE 2011; 6:e16679.

• Teschendorff AE et al. Age-dependent DNA methylation of genes that are suppressed in stem cells is a hallmark of cancer. Genome Res. 2010; 20:440-406.

• Bork S*, Pfister S* et al. DNA Methylation Pattern Changes upon Long-Term Culture and Aging of Human Mesenchymal Stromal Cells. Aging Cell, 2010; 9: 54-63.

• Ho AD, Wagner W, Mahlknecht U. Stem cells and ageing. EMBO Rep, 2005; 6: 35-38.

Analysis of Replicative Senescence

• Göbel C, et al. Interrupted reprogramming into induced pluripotent stem cells does not rejuvenate human mesenchymal stromal cells. Scientific Reports. 2018, 8; 11676.

• Grezella C, et al. Effects of Senolytic Drugs on Human Mesenchymal Stromal Cells. Stem Cell Research & Therapy, 2018, 9: 108.

• Zirkel A, et al. HMGB2 loss upon senescence entry disrupts genomic organization and induces CTCF clustering across cell types. Molecular Cell, 2018; 70: 1-15.

• Franzen J, et al. Senescence-Associated DNA Methylation is Stochastically Acquired in Subpopulations of Mesenchymal Stem Cells. Aging Cell, 2017, 16: 183-191.

• Franzen J, Wagner W, Fernandez-Rebollo E. Epigenetic Modifications upon Senescence of Mesenchymal Stem Cells. Current Stem Cell Reports, 2016; 2: 248–254.

• Wagner W, Fernandez-Rebollo E, Frobel J. DNA-Methylation Changes in Replicative Senescence and Aging: Two Sides of the Same Coin? Epigenomics, 2016 8: 1-3.

• Kalwa M et al. The lncRNA HOTAIR impacts on mesenchymal stem cells via triple helix formation. Nucleic Acid Research 2016, 44: 10631-10643.

• Hänzelmann S et al. Replicative Senescence is Associated with Nuclear Reorganization and with DNA Methylation at Specific Transcription Factor Binding Sites. Clinical Epigenetics 2015; 7:19.

• Schellenberg et al. Proof of principle: quality control of therapeutic cell preparations using senescence-associated DNA-methylation changes. BMC Res Notes, 2014; 7: 254.

• Frobel J et al. Epigenetic Rejuvenation of Mesenchymal Stromal Cells Derived from Induced Pluripotent Stem Cells. Stem Cell Reports. 2014;3:414-22.

• Schellenberg A et al. Matrix Elasticity, Replicative Senescence and DNA Methylation Patterns of Mesenchymal Stem Cells. Biomaterials. 2014;35:6351-8.

• Koch C, Wagner W. Epigenetic Biomarker to Determine Replicative Senescence of in vitro-Cultured Cells. Methods in Molecular Biology 2013; 1048:309-321.

• Koch CM et al. Pluripotent Stem Cells Escape From Senescence-Associated DNA Methylation Changes. Genome Research, 2013; 23: 248-259.

• Walenda G et al. TGF-beta1 does not induce senescence of multipotent mesenchymal stromal cells and has similar effects in early and late passages. PLoS ONE, 2013; 8:e77656.

• Koch C et al. Monitoring of cellular aging by DNA-methylation at specific CpG sites. Aging Cell 2012; 11:366-369.

• Schellenberg A et al. Replicative senescence of mesenchymal stem cells causes DNA-methylation changes which correlate with repressive histone marks. Aging 2011, 3: 873-888.

• Wagner W, Ho AD, Zenke M. The Many Facets of Cellular Aging in Mesenchymal Stromal Cells. Tissue Eng Part B Rev. 2010; 16: 445-453.

• Wagner W. Senescence is heterogeneous in mesenchymal stromal cells - kaleidoscopes for cellular aging. Cell Cycle 2010; 9:2923-2924.

• Wagner W. Implications of long-term culture for mesenchymal stem cells: genetic defects or epigenetic regulation? Stem Cell Research & Therapy 2012; 3: 54.

• Wagner W et al. How to track aging of mesenchymal stromal cells? Aging 2010; 2:224-230.

• Bork S*, Pfister S* et al. DNA Methylation Pattern Changes upon Long-Term Culture and Aging of Human Mesenchymal Stromal Cells. Aging Cell 2010; 9: 54-63.

• Wagner W et al. Aging and replicative senescence have related effects on human stem and progenitor cells. PLoS One, 2009; 4: e5846.

• Wagner W et al. Replicative Senescence of mesenchymal stem cells - a continuous and organized process. PLoS ONE, 2008; 3: e2213.

Epigenetic diagnostics

• Jost E et al. Epimutations Mimic Genomic Mutations of DNMT3A in Acute Myeloid Leukemia. Leukemia, 2014; 28:1227-34.

• Wagner W et al. Do age-associated DNA methylation changes increase the risk of malignant transformation? Bioessays. 2015; 37:20-24.

• Weidner CI et al. Hematopoietic Stem and Progenitor Cells Acquire Distinct DNA-Hypermethylation During in vitro Culture. Scientific Reports, 2013; 3:3372.

• Bozic T*, Lin Q*, et al. DNA-Methylation in C1R is a Prognostic Biomarker for Acute Myeloid Leukemia. Clinical Epigenetics. 2015; 7:116.

• Weidner CI et al. DNA methylation in PRDM8 is indicative for dyskeratosis congenita. Oncotarget, 2016; DOI: 10.18632/oncotarget.7458.

Quality control of cell culture

• Goetzke R, et al. Does Soft Really Matter? Differentiation of Induced Pluripotent Stem Cells into Mesenchymal Stromal Cells is Not Influenced by Soft Hydrogels. Biomaterials, 2018, 156: 147-158.

• Wagner W et al. Epigenetic quality check - how good are your mesenchymal stromal cells? Epigenomics. 2016; 8: 889-894.

• de Almeida DC et al. Epigenetic classification of human mesenchymal stromal cells. Stem Cell Reports 2016; 6: 168-175.

• Lenz M*, Goetzke R* et al. Epigenetic Biomarker to Support Classification into Pluripotent and Non-Pluripotent Cells. Scientific Reports 2015; 5, 8973

• Shao K et al. Induced pluripotent mesenchymal stromal cell clones retain donor-derived differences in DNA methylation profiles. Molecular Therapy 2013; 21:240-250.