From bone marrow or adipose tissue?
Mesenchymal stromal cells (MSCs) can be isolated from various different tissues, such as bone marrow (BM) or adipose tissue (AT). There is a growing perception that MSCs isolated from these two tissues differ significantly in their differentiation potential, hematopoiesis supportive function, and immunomodulatory potential – although they look phenotypically very similar. Our Epi-Tissue-Score enables retrospective analysis of the tissue of isolation.
How does it work?
We identified two CpG dinucleotides – associated with the genes SLC41A2 and TM4SF1 – that are either methylated in MSCs from bone marrow or adipose tissue, respectively. The Epi-Tissue-Score is determined as the difference of these DNAm levels: a level above 0 is indicative for MSCs from bone marrow. We generated pyrosequencing assays that facilitate fast and cost-effective analysis of DNAm levels at the two relevant CpGs (SLC41A2 and TM4SF1 ).
Patent application: 2016; EP16152198.4
Figure 1: Classification by the Epi-Tissue-Score. MSCs from bone marrow (BM) and adipose tissue (AT) were correctly classified by our pyrosequencing assays.